Enzymes in Nonaqueous Solvents

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Format: Hardcover
Pub. Date: 2001-04-01
Publisher(s): Humana Pr Inc
List Price: $199.99

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Summary

In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design. Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.

Table of Contents

Preface v
Contributors xiii
Part I Control of Enzyme Activity in Nonaqueous Solvents 1(240)
Peter J. Halling
Salt-Induced Activation of Enzymes in Organic Solvents: Optimizing the Lyophilization Time and Water Content
3(10)
Michael T. Ru
Jonathan S. Dordick
Jeffrey A. Reimer
Douglas S. Clark
Imprinting Enzymes for Use in Organic Media
13(6)
Joseph O. Rich
Jonathan S. Dordick
Entrapment of Biocatalysts by Prepolymer Methods
19(12)
Atsuo Tanaka
Takamitsu Iida
Microencapsulation of Enzymes and Cells for Nonaqueous Biotransformations
31(10)
Jeffrey A. Khan
Evgeny N. Vulfson
Immobilization of Lipases on Hydrogels
41(8)
Abu Bakar Salleh
Norhaizan M. Esa
Mahiran Basri
Che Nyonya A. Razak
Wan Md Zin W. Yunus
Mansor Ahmad
Polyethylene Glycol-Modified Enzymes in Hydrophobic Media
49(16)
Ayako Matsushima
Yoh Kodera
Misao Hiroto
Hiroyuki Nishimura
Yuji Inada
Chemical Modification of Lipase for Use in Ester Synthesis
65(12)
Mahiran Basri
Kamaruzaman Ampon
Che Nyonya A. Razak
Abu Bakar Salleh
Preparation and Properties in Organic Solvents of Noncovalent PEG-Enzyme Complexes
77(6)
Francesco Secundo
Gianluca Ottlina
Giacomo Carrea
Preparation of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media
83(14)
Toshiaki Mori
Yoshio Okahata
Very High Activity Biocatalysts for Low-Water Systems: Propanol-Rinsed Enzyme Preparations
97(8)
Barry D. Moore
Johann Partridge
Peter J. Halling
Methods for Measurement and Control of Water in Nonaqueous Biocatalysis
105(22)
George Bell
Peter J. Halling
Lindsey May
Barry D. Moore
Donald A. Robb
Rein Ulijn
Rao H. Valivety
Water Activity Control in Organic Media by Equilibration Through Membranes
127(8)
Ernst Wehtje
Patrick Adlercreutz
Water Activity Control for Lipase-Catalyzed Reactions in Nonaqueous Media
135(16)
Joon Shick Rhee
Seok Joon Kwon
Jeong Jun Han
Immobilization of Enzymes and Control of Water Activity in Low-Water Media: Properties and Applications of Celite R-640 (Celite Rods)
151(22)
Lucia Gardossi
Enzyme Activity and Enantioselectivty Measurements in Organic Media
173(10)
Amelie Ducret
Michael Trani
Robert Lortie
Calorimetric Methods in Evaluating Hydration and Solvation of Solid Proteins Immersed in Organic Solvents
183(20)
Mikhail Borisover
Vladimir Sirotkin
Dmitriy Zakharychev
Boris Solomonov
Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopy
203(10)
Hideo Kise
The Effects of Crown Ethers on the Activity of Enzymes in Organic Solvents
213(14)
Dirk-Jan van Unen
Johan F. J. Engbersen
David N. Reinhoudt
Control of Acid-Base Conditions in Low-Water Media
227(8)
Johann Partridge
Neil Harper
Barry D. Moore
Peter J. Halling
Enzymatic Acylation of α-Butylglucoside in Nonaqueous Media
235(6)
Marie-Pierre Bousquet
Rene-Marc Willemot
Pierre Monsan
Emmanuel Boures
Part II Synthetic Applications 241(228)
Herbert L. Holland
Choosing Hydrolases for Enantioselective Reactions Involving Alcohols Using Empirical Rules
243(18)
Alexandra N. E. Weissfloch
Romas J. Kazlauskas
Candida antarctica Lipase B: A Tool for the Preparation of Optically Active Alcohols
261(16)
Didier Rotticci
Jenny Ottosson
Torbjorn Norin
Karl Hult
Enantioselective Lipase-Catalyzed Transesterifications in Organic Solvents
277(14)
Fritz Theil
Pseudomonas cepacia Lipase-Catalyzed Enantioselective Acylation of 2-Substituted-1-alkanols in Organic Solvents
291(16)
Patrizia Ferraboschi
Enzo Santaniello
Preparation of 2-, 3-, and 4-Methylcarboxylic Acids and the Corresponding Alcohols of High Enantiopurity by Lipase-Catalyzed Esterification
307(12)
Per Berglund
Erik Hedenstrom
Optimization of Enzymatic Enantiometric Resolutions Through Solvent Selection
319(6)
Gianluca Ottlina
Francesco Secundo
Giorgio Colombo
Giacomo Carrea
Chemoselective Amidification of Amino-Polyols Catalyzed with Lipases in Organic Solvents
325(6)
Thierry Maugard
Magali Remaud-Simeon
Pierre Monsan
Synthesis of Esters Catalyzed by Lipases in Water-in-Oil Microemulsions
331(8)
Haralambos Stamatis
Aristotelis Xenakis
Fragiskos N. Kolisis
Enzymatic Conversion of Organosilicon Compounds in Organic Solvents
339(18)
Takuo Kawamoto
Atsuo Tanaka
Synthetic Applications of Enzymes in Nonaqueous Media
357(16)
Valerie Rolland
Rene Lazaro
Enzymes in Nonaqueous Solvents: Applications in Carbohydrate and Peptide Preparation
373(28)
Shui-Tein Chen
Boonyaras Sookkheo
Suree Phutrahul
Kung-Tsung Wang
Interface Bioreactor: Microbial Transformation Device on an Interface Between a Hydrophilic Carrier and a Hydrophobic Organic Solvent
401(16)
Shinobu Oda
Takeshi Sugai
Hiromichi Ohta
Yeast-Mediated Reactions in Organic Solvents
417(6)
Andrew J. Smallridge
Maurie A. Trewhella
Biocatalysis in Pharmaceutical Process Development: SCH56592, a Case Study
423(46)
Brian Morgan
David R. Dodds
Michael J. Homann
Aleksey Zaks
Robert Vail
Part III Reaction Systems and Bioreactor Design 469(182)
Evgeny N. Vulfson
Enzymatic Solid-to-Solid Peptide Synthesis
471(8)
Markus Erbeldinger
Uwe Eichhorn
Peter Kuhl
Peter J. Halling
Enzymatic Synthesis and Hydrolysis Reactions of Acylglycerols in Solvent-Free Systems
479(18)
Cristina Otero
Jose A. Arcos
Hugo S. Garcia
Charles G. Hill, Jr.
Solid--Gas Catalysis at Controlled Water Activity: Reactions at the Gas--Solid Interface Using Lipolytic Enzymes
497(12)
Sylvain Lamare
Marie Dominique Legoy
Solvent-Free Biotransformations of Lipids
509(8)
Tsuneo Yamane
Lipase-Catalyzed Synthesis of Sugar Fatty Acid Esters in Supercritical Carbon Dioxide
517(6)
Haralambos Stamatis
Vasiliki Sereti
Fragiskos N. Kolisis
Transformations in Frozen Aqueous Solutions Catalyzed by Hydrolytic Enzymes
523(8)
Marion Haensler
Hans-Dieter Jakubke
Enzymatic Synthesis of Sugar Fatty Acid Esters in Solvent-Free Media
531(14)
Douglas B. Sarney
Evgeny N. Vulfson
Biotransformations in Supersaturated Solutions
545(8)
David A. MacManus
Anna Millqvist-Fureby
Evgeny N. Vulfson
Enzymatic Transformations in Suspensions (I): One Solid Substrate and Product
553(12)
Volker Kasche
Antje Spieb
Biotransformations in Supercritical Fluids
565(10)
Nuno Fontes
M. Conceicao Almeida
Susana Barreiros
Reverse Micellar Systems: General Methodology
575(12)
Andrey V. Levashov
Natalia L. Klyachko
Enzymatic Transformations in Supercritical Fluids
587(16)
Alain Marty
Jean-Stephane Condoret
Enzymatic Transformations in Supensions (II)
603(8)
Adrie J. J. Straathof
Mike J. J. Litjens
Joseph J. Heijnen
Characterization and Operation of a Micellar Membrane Bioreactor
611(16)
Cristina M. L. Carvalho
Maria Raquel Aires-Barros
Joaquim M. S. Cabral
Immobilization of Lipase Enzymes and Their Application in the Interesterification of Oils and Fats
627(24)
Alan D. Peilow
Maha M. A. Misbah
Index 651

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