Preface |
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v | |
Contributors |
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xiii | |
Part I Control of Enzyme Activity in Nonaqueous Solvents |
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1 | (240) |
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Salt-Induced Activation of Enzymes in Organic Solvents: Optimizing the Lyophilization Time and Water Content |
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3 | (10) |
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Imprinting Enzymes for Use in Organic Media |
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13 | (6) |
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Entrapment of Biocatalysts by Prepolymer Methods |
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19 | (12) |
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Microencapsulation of Enzymes and Cells for Nonaqueous Biotransformations |
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31 | (10) |
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Immobilization of Lipases on Hydrogels |
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41 | (8) |
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Polyethylene Glycol-Modified Enzymes in Hydrophobic Media |
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49 | (16) |
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Chemical Modification of Lipase for Use in Ester Synthesis |
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65 | (12) |
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Preparation and Properties in Organic Solvents of Noncovalent PEG-Enzyme Complexes |
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77 | (6) |
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Preparation of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media |
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83 | (14) |
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Very High Activity Biocatalysts for Low-Water Systems: Propanol-Rinsed Enzyme Preparations |
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97 | (8) |
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Methods for Measurement and Control of Water in Nonaqueous Biocatalysis |
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105 | (22) |
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Water Activity Control in Organic Media by Equilibration Through Membranes |
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127 | (8) |
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Water Activity Control for Lipase-Catalyzed Reactions in Nonaqueous Media |
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135 | (16) |
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Immobilization of Enzymes and Control of Water Activity in Low-Water Media: Properties and Applications of Celite R-640 (Celite Rods) |
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151 | (22) |
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Enzyme Activity and Enantioselectivty Measurements in Organic Media |
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173 | (10) |
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Calorimetric Methods in Evaluating Hydration and Solvation of Solid Proteins Immersed in Organic Solvents |
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183 | (20) |
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Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopy |
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203 | (10) |
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The Effects of Crown Ethers on the Activity of Enzymes in Organic Solvents |
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213 | (14) |
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Control of Acid-Base Conditions in Low-Water Media |
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227 | (8) |
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Enzymatic Acylation of α-Butylglucoside in Nonaqueous Media |
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235 | (6) |
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Part II Synthetic Applications |
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241 | (228) |
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Choosing Hydrolases for Enantioselective Reactions Involving Alcohols Using Empirical Rules |
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243 | (18) |
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Alexandra N. E. Weissfloch |
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Candida antarctica Lipase B: A Tool for the Preparation of Optically Active Alcohols |
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261 | (16) |
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Enantioselective Lipase-Catalyzed Transesterifications in Organic Solvents |
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277 | (14) |
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Pseudomonas cepacia Lipase-Catalyzed Enantioselective Acylation of 2-Substituted-1-alkanols in Organic Solvents |
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291 | (16) |
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Preparation of 2-, 3-, and 4-Methylcarboxylic Acids and the Corresponding Alcohols of High Enantiopurity by Lipase-Catalyzed Esterification |
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307 | (12) |
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Optimization of Enzymatic Enantiometric Resolutions Through Solvent Selection |
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319 | (6) |
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Chemoselective Amidification of Amino-Polyols Catalyzed with Lipases in Organic Solvents |
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325 | (6) |
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Synthesis of Esters Catalyzed by Lipases in Water-in-Oil Microemulsions |
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331 | (8) |
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Enzymatic Conversion of Organosilicon Compounds in Organic Solvents |
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339 | (18) |
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Synthetic Applications of Enzymes in Nonaqueous Media |
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357 | (16) |
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Enzymes in Nonaqueous Solvents: Applications in Carbohydrate and Peptide Preparation |
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373 | (28) |
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Interface Bioreactor: Microbial Transformation Device on an Interface Between a Hydrophilic Carrier and a Hydrophobic Organic Solvent |
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401 | (16) |
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Yeast-Mediated Reactions in Organic Solvents |
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417 | (6) |
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Biocatalysis in Pharmaceutical Process Development: SCH56592, a Case Study |
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423 | (46) |
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Part III Reaction Systems and Bioreactor Design |
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469 | (182) |
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Enzymatic Solid-to-Solid Peptide Synthesis |
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471 | (8) |
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Enzymatic Synthesis and Hydrolysis Reactions of Acylglycerols in Solvent-Free Systems |
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479 | (18) |
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Solid--Gas Catalysis at Controlled Water Activity: Reactions at the Gas--Solid Interface Using Lipolytic Enzymes |
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497 | (12) |
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Solvent-Free Biotransformations of Lipids |
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509 | (8) |
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Lipase-Catalyzed Synthesis of Sugar Fatty Acid Esters in Supercritical Carbon Dioxide |
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517 | (6) |
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Transformations in Frozen Aqueous Solutions Catalyzed by Hydrolytic Enzymes |
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523 | (8) |
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Enzymatic Synthesis of Sugar Fatty Acid Esters in Solvent-Free Media |
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531 | (14) |
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Biotransformations in Supersaturated Solutions |
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545 | (8) |
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Enzymatic Transformations in Suspensions (I): One Solid Substrate and Product |
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553 | (12) |
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Biotransformations in Supercritical Fluids |
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565 | (10) |
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Reverse Micellar Systems: General Methodology |
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575 | (12) |
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Enzymatic Transformations in Supercritical Fluids |
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587 | (16) |
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Enzymatic Transformations in Supensions (II) |
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603 | (8) |
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Characterization and Operation of a Micellar Membrane Bioreactor |
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611 | (16) |
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Maria Raquel Aires-Barros |
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Immobilization of Lipase Enzymes and Their Application in the Interesterification of Oils and Fats |
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627 | (24) |
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Index |
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651 | |